Anti-Müllerian hormone (AMH), a glycoprotein secreted from the fetal testis, is responsible for regression of the Müllerian duct in the male fetus. The aim of this study was to evaluate the usefulness of serum AMH as a biomarker for diagnosis of cryptorchidism in horses. Serum AMH concentrations were measured in intact stallions, hemi-castrated unilateral cryptorchid stallions, and geldings. In addition, expression of AMH was characterized in cryptorchid testes by immunohistochemistry. Serum AMH was detected in intact stallions (n=11, 13.3 ± 1.8 ng/ml) and in hemi-castrated cryptorchid stallions (n=8, 17.6 ± 3.0 ng/ml), but not in geldings (n=6, all data were below the limit of detection). Immunolabeling for AMH was detected in Sertoli cells of undescended testes from cryptorchid horses as well as those of normal testes. Our findings indicate that the cryptorchid testis after hemi-castration secretes AMH and that serum AMH concentrations may be a useful biomarker for diagnosis of equine cryptorchidism.
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机译：抗苗勒氏管激素（AMH）是胎儿睾丸分泌的一种糖蛋白，可导致雄性胎儿苗勒氏管退化。这项研究的目的是评估血清AMH作为诊断马匹隐睾症的生物标志物的有用性。在完整的种马，半-割的单侧隐睾种马和gel中测量血清AMH浓度。此外，通过免疫组织化学表征了隐睾睾丸中AMH的表达。在完整的种马（n = 11，13.3±1.8 ng / ml）和半cast割的隐睾种马（n = 8，17.6±3.0 ng / ml）中检测到血清AMH，但在胶凝物中（n = 6，所有数据）未检测到低于检出限）。在隐睾马和正常睾丸的未降睾丸的睾丸支持细胞中检测到AMH的免疫标记。我们的发现表明，半cast割后的隐睾睾丸会分泌AMH，并且血清AMH浓度可能是诊断马隐睾症的有用生物标记。
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